Figure 3. Mis-expression of Ato^Dm and Ato^Bm induces related phenotypes in the wing and the eye.

A) Mis-expression Ato^Dm or Ato^Bm in the developing wing disc under the control of dpp-Gal4 induces formation of ectopic chordotonal organs (arrow) along the 3^rd wing vein. Lower panel shows high magnification of region marked in upper panel. B) Mis-expression Ato^Dm or Ato^Bm under the control of pGMR-Gal4 disrupts eye development leading to the formation of a ‘rough’ adult eye in which the spatial arrangement of the ommatidia is disrupted. Developing disc corresponding to the adult eyes shown above were stained for Sens to mark R8 neurons, the pan-neural marker Elav to highlight all photoreceptors and the retina-identity determinant Eya to mark the developing eye disc. Single channels for Sens and Elav are shown below. Misexpression of Ato^Dm or Ato^Bm results in the formation of supernumerary R8 neurons (Sens-positive). In both wing and eye, Ato^Dm has a stronger effect than Ato^Bm but the resulting phenotypes are consistent with a similar activity of the two proteins in these experiments.