Table 2. SSRs in non-redundant white clover ESTs and designed EST-SSR primers.
| SSR Pattern | SSR Numbers in Non-redundant White Clover ESTs | Frequency (%) | Designed EST-SSR Primers | |||
|---|---|---|---|---|---|---|
| Mismatch 0 | Mismatch 1 | Mismatch 2 | Total | |||
| AG | 211 | 16.67 | 30 | 19 | 44 | 93 |
| AT | 22 | 1.74 | 7 | 3 | 15 | 25 |
| AC | 8 | 0.63 | 4 | 6 | 11 | 21 |
| GC | 0 | 0.00 | 0 | 0 | 0 | 0 |
| AAC | 182 | 14.38 | 49 | 64 | 139 | 252 |
| AAG | 179 | 14.14 | 35 | 90 | 246 | 371 |
| GGT | 170 | 13.43 | 27 | 63 | 157 | 247 |
| ATC | 113 | 8.93 | 30 | 49 | 151 | 230 |
| AAT | 86 | 6.79 | 17 | 21 | 66 | 104 |
| AGC | 79 | 6.24 | 8 | 33 | 90 | 131 |
| GGA | 36 | 2.84 | 10 | 34 | 89 | 133 |
| GGC | 19 | 1.50 | 2 | 8 | 47 | 57 |
| ACT | 16 | 1.26 | 5 | 12 | 21 | 38 |
| ACG | 4 | 0.32 | 0 | 5 | 21 | 26 |
| AATG | 26 | 2.05 | 3 | 12 | 19 | 34 |
| AAAT | 23 | 1.82 | 4 | 13 | 30 | 47 |
| AAAG | 20 | 1.58 | 3 | 5 | 44 | 52 |
| AGTG | 8 | 0.63 | 0 | 0 | 0 | 0 |
| ATAG | 8 | 0.63 | 0 | 0 | 0 | 0 |
| AATT | 8 | 0.63 | 3 | 2 | 17 | 22 |
| ATAC | 5 | 0.39 | 0 | 0 | 0 | 0 |
| AACC | 4 | 0.32 | 0 | 0 | 0 | 0 |
| AAAC | 4 | 0.32 | 0 | 9 | 34 | 43 |
| AACT | 3 | 0.24 | 0 | 0 | 0 | 0 |
| Other tetra-nucleotide repeats | 32 | 2.53 | 4 | 8 | 35 | 47 |
| Total | 1266 | 100 | 241 | 456 | 1276 | 1973 |