Figure 4.

Effect of dietary cinchonine on the expression of genes involved in inflammation. Mice were fed on the ND, HFD, or CID. (a) RT-PCR analyses of TLRs-mediated proinflammatory cytokine genes. The data shown are the relative density normalized to GAPDH. (b) Protein levels of phosphorylation of IRF3 (p-IRF3) and total IRF3 in the epididymal adipose tissue of mice analyzed by Western blot. p-IRF3 was normalized to total IRF3 protein level. Bars represent the mean ± SEM, n = 8, P < 0.05. Different letters are statistically different.