Table 1.
Primer sequences and PCR conditions.
| Gene description | Primers | Sequences (5′→3) | Annealing temperature(°C) |
PCR product (bp) |
|---|---|---|---|---|
| Peroxisome proliferator-activated receptor gamma2 (PPARγ2) | F R |
TTCGGAATCAGCTCTGTGGA CCATTGGGTCAGCTCTTGTG |
55 | 148 |
| CCAAT/enhancer-binding protein, alpha (C/EBPα) | F R |
AAGGCCAAGAAGTCGGTGGA CCATAGTGGAAGCCTGATGC |
55 | 189 |
| Adipocyte protein 2 (aP2) | F R |
ACATGAAAGTGGGAGTG AAGTACTCTCTGACCGGATG |
55 | 128 |
| Leptin | F R |
CTCCAAGGTTGTCCAGGGTT AAAACTCCCCACAGAATGGG |
55 | 143 |
| Sterol regulatory element-binding factor 1 (SREBP1c) | F R |
TTGTGGAGCTCAAAGACCTG TGCAAGAAGCGGATGTAGTC |
55 | 94 |
| Lipoprotein lipase (LPL) | F R |
TGCCGCTTTTTGTTTTACC TCACAGTTTCTGCTCCCAGC |
55 | 172 |
| Secreted frizzled-related protein 5 (SFRP5) | F R |
CTTGGTGTCCTTGCGCTTTA CTGATGGCCTCATGGAACAG |
61 | 155 |
| Wingless-type MMTV integration site family, member 10B (WNT10b) | F R |
TTTTGGCCACTCCTCTTCCT TCCTTTTCCAACCGAAAACC |
61 | 183 |
| Dickkopf 2 (DKK2) | F R |
GCATTTCCTTCAGATTGGCA TCATTCCCTGTTCTTCAGCG |
55 | 144 |
| Galanin receptor 1 (GalR1) | F R |
CCAAGGGGGTATCCCAGTAA GGCCAAACA CTACCAGCGTA |
55 | 147 |
| Galanin receptor 2 (GalR2) | F R |
ATAGTGGTGCTCATGCTGGAA AGGCTGGATCGAGGGTTCTA |
60 | 134 |
| Protein kinase C δ (PKCδ) | F R |
CTGAGCGCTGCAAGAAGAAC TGGAAACTTTGATCCTGCACTGA |
60 | 146 |
| Cyclin D (Cyc-D) | F R |
TGGGAAGTTTTGTTGGGTCA TCCTTGTCCAGGTAATGCCA |
55 | 144 |
| E2F1 | F R |
CCTGGAGCATGTTAAAGAAG CCTCGAGACCAAAGTGATAG |
48.9 | 102 |
| Toll-like receptor 2 (TLR2) | F R |
TCTAAAGTCGATCCGCGACA TACCCAGCTCGCTCACTACG |
56.2 | 344 |
| Toll-like receptor 4 (TLR4) | F R |
ACCTCTGCCTTCACTACAGA AGGGACTTCTCAACCTTCTC |
48.6 | 223 |
| Myeloid differentiation primary response gene 88 (MyD88) | F R |
AAGAAAGTGAGTCTCCCCTC TCCCATGAAACCTCTAACAC |
55 | 149 |
| Toll-interleukin 1 receptor domain-containing adaptor protein (Tirap) | F R |
GTGGCCGCTGGAGCAAAGAC TTGCCTCTGCCATCCACATA |
55 | 370 |
| TNF receptor-associated factor 6 (TRAF6) | F R |
GCACAAGTGCCCAGTTGACA AGTGTCGTGCCAAGTGATTC |
62.7 | 479 |
| TIR-domain-containing adapter-inducing interferon-β (TRIF) | F R |
ATGGATAACCCAGGGCCTT TTCTGGTCACTGCAGGGGAT |
56.5 | 528 |
| Interferon regulatory factor 5 (IFR5) | F R |
AATACCCCACCACCTTTTGA TTGAGATCCGGGTTTGAGAT |
53.1 | 191 |
| Interferon α (IFNα) | F R |
ATGGCTAG(G/A)CTCTGTGCTTTCCT GGGCTCTCCAGA(T/C)TTCTGCTCTG |
60.2 | 500 |
| Tumor necrosis factor alpha (TNFα) | F R |
TGTCTCAGCCTCTTCTCATT AGATGATCTGAGTGTGAGGG |
55 | 156 |
| Intereukin 6 (IL-6) | F R |
TTGCCTTCTTGGGACTGATG CCACGATTTCCCAGAGAACA |
55 | 162 |
| Forkhead box transcription factor O1 (FOXO1) | F R |
TCCCAATGGCACAGTCCTTA AGCAGTCCAAAGATGCCCTT |
55.2 | 185 |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F R |
AGAACATCATCCCTGCATCC TCCACCACCCTGTTGCTGTA |
60 | 321 |