Figure 4.

Increased random cell motility alters pattern formation in multicellular aggregates of cultured VMCs from labyrinths to periodic holes. (a–c) Self-organized VMC pattern in (a) normal condition, or with (b) ROCK inhibition or (c) NMM-II inhibition. Aggregates were stained with haematoxylin. Insets in (a), higher magnification of multicellular aggregates. Scale bar, 2 mm and 300 µm (insets). (d–f) Computational simulations showing n(x, y) as (d) a labyrinthine pattern reflecting the normal culture condition (D_n = 0.06) and periodic holes (e) reflecting ROCK inhibition (D_n = 0.6) and (f) NMM-II inhibition (D_n = 0.2).