Figure 6.

DISC1 does not affect nuclear targeting and protein levels of ATF4. (A) Representative COS7 cells transfected with ATF4 alone (top panels) or in combination with DISC1 (lower panels). The area delimited by a white rectangle is magnified in the inset. (B) ATF4 protein levels in whole-cell lysates or nuclear extracts prepared from HEK293 cells transfected with ATF4 alone or in combination with DISC1. The ATF4/DISC1 DNA ratio used in these experiments was the same as that used for the luciferase reporter assays. Loading controls are histone 3 (H3) for whole-cell lysates and nuclear matrix protein p84 for nuclear extracts. The bars represent the average of three independent experiments. Scale bars are 20 μm. The position and size (kDa) of the protein markers is indicated.