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. 2012 Mar 15;21(12):2779–2792. doi: 10.1093/hmg/dds106

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© The Author 2012. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Effect of DISC1 variants on the interaction between DISC1 and ATF4. (A) HEK293 cells were transfected with pCG-ATF4 alone or in combination with the indicated DISC1variants. Exogenous DISC1 was immunoprecipitated using an anti-Flag antibody and detected using an anti-DISC1 antibody (α-DISC1). Co-precipitating ATF4 was detected with an anti-ATF4 antibody (sc-200). The bars represent the average of three independent experiments. *Non-specific band. The position and size (kDa) of the protein markers is indicated. (B) COS7 cells transfected with the indicated variants of DISC1 (green) and ATF4 (red). The right-hand panels represent the corresponding merged images. The areas delimited by a white square are magnified in the insets. Scale bars are 20 μm.