Figure 4.
Baseline motor activity and habituation, monitored via the open-field activity test, is not affected in ERK5 icKO mice. A, Schematic illustration of the experimental design. Tamoxifen or vehicle was administered to 12-week-old Nestin-CreER™/ERK5loxP/loxP mice orally, 4 d in each cycle, for a total of three cycles with 2 week intercycle intervals. Animals were subjected to behavior assays 2 weeks after the last dose of tamoxifen. B, Total floor plane movement on day 1 (d1), d2, and d3. C, Total time spent along the margins of the open field. D, Total distance traveled. E, Average traveling speed. Data represent the mean of two independent experiments with n ≥ 10 animals per treatment group per experiment.