Table 1. Calculated and Observed Monoisotopic Molecular Weights of Oligonucleotides and Intermediates Present in the AlkB Reactions.
| lesion or base | MW (calculated) of neutral species | m/z (calculated) –4 charge monoisotopic peak | m/z (observed) –4 charge monoisotopic peak |
|---|---|---|---|
| A | 4888.86 | 1221.21 | 1221.13/16/24 |
| m1A | 4902.88 | 1224.71 | 1224.67 |
| eA | 4912.86 | 1227.21 | 1227.22 |
| eA epoxide | 4928.86 | 1231.21 | 1231.22 |
| eA glycol | 4946.87 | 1235.71 | 1235.72 |
| EA | 4914.88 | 1227.71 | 1227.67 |
| EA+2 | 4916.86 | 1228.21 | 1228.16 |
| EA+16 | 4930.87 | 1231.71 | 1231.66 |
| EA+32 | 4946.87 | 1235.71 | 1235.67 |
| m6A | 4902.88 | 1224.71 | 1224.73 |
| HO-m6A | 4918.87 | 1228.71 | 1228.72 |