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. Author manuscript; available in PMC: 2012 May 30.
Published in final edited form as: J Control Release. 2010 Jan 28;144(1):101–108. doi: 10.1016/j.jconrel.2010.01.021

Figure 4.

Figure 4

Noggin loaded and released from HGC maintains its biological activity. (A) HGC-Noggin maintains the ability to form dimers as determined by Noggin WIB under non-reducing conditions. (S0): native Noggin; (S4): Noggin loaded to HGC. (B) Noggin released from HGC inhibits Smad-1,5,8 phosphorylation. HGC-Noggin, HGC-BSA, HGC alone were incubated for 48 hours in SF medium that was collected, dialyzed and used to treat limb mesenchyme micromasses for 20 minutes. a, control medium; b, medium from HGC alone; c, medium from HGC-BSA; d, medium from HGC-Noggin; e, 200 ng of native Noggin