Table 1.

 Polymerase chain reaction primer sets and sequencing primers used for ABL1 kinase domain mutation quantification by pyro‐sequencing

PCR set	Codons	Common mutations	PCR primers†	Sequencing primers
1	236–274	M244V, L248V, G250E, Q252H, Y253H, E255V	F: 5′‐AGATGGAACGCACGGACAT‐3′ R: 5′‐B‐CTTCAAGGTCTTCACGGC‐3′	5′‐AGATGGAACGCACGGACAT‐3′ (same as F)
2	300–349	F311I, T315I, F317L	F: 5′‐AGCTCCTTGGGGTCTGCA‐3′ R: 5′‐B‐TGACGAGATCTGAGTGGC‐3′	5′‐AGCTCCTTGGGGTCTGCA‐3′ (same as F)
3	343–392	M351T, E355G, E355A, F359C, F359V	F: 5′‐TGGCCACTCAGATCTCGTC‐3′ R: 5′‐B‐TGTCCCCTGTCATCAACCT‐3′	5′‐TGGCCACTCAGATCTCGTC‐3′ (same as F)
4	343–422	L364I, H396R	F: 5′‐TGGCCACTCAGATCTCGTC‐3′ R: 5′‐B‐ACGTCGGACTTGATGGAG‐3′	5′‐ATGACAGGGGACACCTACAC‐3′ (for H396R only)

†The primers listed are for second‐round PCR. F, forward primer; R, reverse primer; B, biotin‐tagged.