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. Author manuscript; available in PMC: 2013 May 24.
Published in final edited form as: J Med Chem. 2012 May 16;55(10):4640–4651. doi: 10.1021/jm300460c

Figure 5.

Figure 5

Influence of 2-mercaptoethanol for the inhibition of the VDR-SRC2-3 binding in the presence of 3-indolyl-methanamine, 31b. VDR-LBD (1 μM), LG190178 (5 μM), and Alexa Fluorlabeled SRC2-3 peptide (7 nM) were incubated in the presence of different concentrations of 31b and in the absence and presence of different concentrations of 2-mercaptoethanol. Interactions between VDR and SRC2-3 were determined by fluorescence polarization. ○ DMSO (negative control), □ 3- dibutylamino-1-(4-hexyl-phenyl)-propan-1-one (positive control), 2-mercaptoethanol concentrations (31b IC50 values): ● 100 mM (82.2 ± 7.3 μM), ■ 10 mM (54.5 ± 3.1 μM), ▲ 1 mM (45.4 ± 2.0 μM), ▼ 0.1 mM (37.5 ± 1.1 μM), ◆ 0.01 mM (36.8 ± 0.5 μM).