TABLE 3:

Chemomechanical properties of PwMhc2 (control) and mutant (E701K) myosin.

Myosin type	CaATPase ± SD (s−1)	Basal MgATPase ± SD (s−1)	Actin-stimulated Vmax ± SD (s−1)	Km (actin) ± SD (μM)	Catalytic efficiency Vmax/Km ± SD	Actin sliding velocity ± SD (μm s−1)
PwMhc2 (control)	10.35 ± 1.14 (n = 8)	0.25 ± 0.02 (n = 8)	1.84 ± 0.32 (n = 8)	0.25 ± 0.09 (n = 8)	7.86 ± 1.84 (n = 8)	6.67 ± 0.97 (n = 6)
E701K-5	0.84 ± 0.36a (n = 5)	0.03 ± 0.01a (n = 5)	0.23 ± 0.06a (n = 5)	0.39 ± 0.11b (n = 5)	0.61 ± 0.16a (n = 5)	1.34 ± 0.05a (n = 4)

^aSignificantly different from control (p < 0.001).

^bSignificantly different from control (p < 0.05).