TABLE 1:
The S. cerevisiae strains used. Continued
| Strain | Genotype | Source/reference |
|---|---|---|
| 23344c | Mata ura3 (Σ1278b genetic background) | Bruno André Université LIbre de Bruxelles, Belgium |
| 27038a | Mata ura3 npi1 (Σ1278b genetic background; mutation in RSP5 promoter) | Bruno André ( Hein et al., 1995) |
| VA029 | Mata ura3 ubp2Δ::KanMX6 (Σ1278b genetic background) | Bruno André |
| BY4741 (WT) | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | EUROSCARF |
| RHT 499 | Matα his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 promKanMX4-RSP5 | Bruno André ( Erpapazoglou et al., 2008) |
| RHT 500 | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubp2Δ::KanMX6 | EUROSCARF |
| RHT 501 (HSE1-3xHA) | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 HSE1-3HA::HIS3MX6 | This study |
| RHT 502 (6xHA-VPS27) | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 6xHA-VPS27 | This study |
| RHT503 (VPS36-3xHA) | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 VPS36-3xHA::HIS3MX6 | This study |
| RHT504 (BRO1-3xHA) | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 BRO1-3xHA::HIS3MX6 | This study |
| RHT505 (HSE1-3xHA/rps5) | Matα his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 promKanMX4-RSP5 HSE1-3xH::HIS3MX6 | This study |
| RHT506 (6xHA-VPS27/rsp5) | Matα his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 promKanMX4-RSP5 6xHA-VPS27 | This study |
| RHT507 (VPS36-3xHA/rps5) | Matα his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 promKanMX4-RSP5 VPS36-3xHA::HIS3MX6 | This study |
| RHT508 (BRO1-3xHA/rsp5) | Matα his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 promKanMX4-RSP5 BRO1-3xHA::HIS3MX6 | This study |
| RHT509 (HSE1-3xHA/ubp2Δ) | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubp2Δ::KanMX6 HSE1-3xHA::HIS3MX6 | This study |
| RHT510 (6xHA-VPS27/ubp2Δ) | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubp2::KanMX6 6xHA-VPS27 | This study |
| RHT511 (VPS23-3xHA/ubp2Δ) | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubp2Δ::KanMX6 VPS23-3xHA::HIS3MX6 | This study |
| RHT512 (VPS36-3xHA/ubp2Δ) | Mata his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubp2Δ::KanMX6 VPS36-3xHA::HIS3MX6 | This study |
| RHT513 (BRO1-3xHA/ubp2Δ) | Mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubp2Δ::KanMX6 BRO1-3xHA::HIS3MX6 | This study |
| SUB280 | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-Ub) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) | Spence et al. (1995) |
| SUB413 | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-UbK63R) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) | Spence et al. (1995) |
| SUB515 | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-UbK6R) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) | Spence et al. (1995) |
| SUB516 | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-UbK11R) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) | Spence et al. (1995) |
| SUB517 | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-UbK27R) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) | Spence et al. (1995) |
| SUB518 | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-UbK29R) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) | Spence et al. (1995) |
| SUB519 | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-UbK33R) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) | Spence et al. (1995) |
| SUB280 vps23Δ | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-Ub) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) vps23Δ::KanMX6 | Helle Ulrich, London Research Institute, UK |
| SUB413 vps23Δ | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3 ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-UbK63R) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) vps23Δ::KanMX6 | Helle Ulrich |
| TDY2 vam3ts | Mata ura3-52 leu2-3112 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 mel GAL vam3Δ::LEU2 + pVAM3ts414 | Darsow et al. (1997) |
| RHT516 (ubp2Δ vam3-ts) | Mata ura3-52 leu2-3112 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 mel ubp2Δ::KanMX6 GAL vam3Δ::LEU2 + pVAM3ts414 | This study |
| RHT514 (SUB280 vam3-ts) | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-Ub) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) vam3Δ::KanMX6 + pRS316-VAM3ts | This study |
| RHT515 (SUB413 vam3-ts) | Mata ubi1-Δ1::TRP1 ubi2-Δ2:ura3, ubi3-Δub-2 ubi4-Δ2::LEU2 (pUB39-UbK63R) (pUB100) lys2-801 leu2-3112 ura3-52 his3-Δ200 trp1-1(am) vam3Δ::KanMX6 + pRS316-VAM3ts | This study |
EUROSCARF, European Saccharomyces cerevisiae Archive for Functional Analysis, Institute for Molecular Biosciences, Johann Wolfgang Goethe-University Frankfurt, Frankfurt, Germany.