TABLE 2:

The plasmids used.

Plasmid	Characteristics, use	Reference
pRS316	CEN, URA3 ( Figure 2)	Sikorski and Hieter (1989)
pSL22	GPD1, CEN, LEU2; expression of Ear1-mCherry ( Figure 1)	Leon et al. (2008)
pGFP-Phm5	TPI1, CEN, URA3; expression of GFP-Phm5 ( Figures 2C and 5A)	Reggiori and Pelham (2001)
pUb-GFP-Phm5-K6R	TPI1, CEN, URA3; expression of Ub(K29,48,63R)-GFP-Phm5-K6R ( Figure 5B)	Reggiori and Pelham (2001)
YEp352-HisUb	CUP1, 2μ, URA3; expression of 6His-Ub ( Figure 6B)	Vitaliano-Prunier et al. (2008)
pVAM3ts414	promVAM3, CEN, TRP1; construction of strains SUB280 vam3ts and SUB413 vam3ts	Darsow et al. (1997)
pRS316-VAM3ts	promVAM3, CEN, URA3 ( Figure 7)	This study
pcDNA3-MART-1	Expression of MART-1 ( Figures 3B and 4)	Levy et al. (2005)
pHR-SIN-CMVp-6His-wt Ub-GFP	Expression of 6His-wtUb-GFP ( Figure 4)	Boname et al. (2010), Tsirigotis et al. (2001)
pHR-SIN-CMVp-6His-UbK63R-GFP	Expression of 6His-UbK63R-GFP ( Figure 4)	Boname et al. (2010), Tsirigotis et al. (2001)
pSIT1-GFP	GAL1, CEN, URA3; expression of Sit1-GFP ( Figure 2B)	Froissard et al. (2007)