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Diffusion coefficients of GFP constructs in the ER estimated using FRAP and FCS (μm2/s ± SEM).
| GFP-LactC2 FRAP (n) | ss-GFP-KDEL FCS (n) | ss-LactC2-GFP-KDEL FCS (n) | ss-LactC2(AAA)-GFP-KDEL FCS (n) | |
|---|---|---|---|---|
| D of the fast component | n.a. | 23.62 ± 1.86 (41) | 18.52 ± 1.51 (30) | 18.54 ±1.51 (35) |
| D of the slow component | 0.79 ± 0.08 (15) | 0.78 ± 0.09 (41) | 0.57 ± 0.04 (30) | 0.53 ± 0.05 (35) |
| Fraction of slow component | n.a. | 32% ± 1% (41) | 48% ± 2% (30) | 33% ± 2% (35) |
