Table 2. Sequence coverages of phage 2972 proteins from virus-enriched and cellular fractions across infection time points.
| Module | Orf | Description, Molecular Weight | Virus Enriched Fraction MOI = 1 | Cellular FractionMOI = 1 | Cellular FractionMOI = 0.1 | |||||||||||||||
| 0 | 0.5 | 1 | 2 | 4 | 24 | 0 | 0.5 | 1 | 2 | 4 | 24 | 0 | 0.5 | 1 | 2 | 4 | 24 | |||
| * | 1 | unknown function, MW:16160 | 65% | 80% | 85% | 69% | 71% | 66% | 63% | 40% | 35% | |||||||||
| Packaging | 2 | terminase small subunit, MW:16777 | 19% | 24% | 25% | 56% | 31% | 85% | 71% | 67% | 81% | 19% | 38% | 25% | 11% | |||||
| 3–4 | terminase large subunit, MW: 47066 | 16% | 43% | 20% | 19% | |||||||||||||||
| Capsid Morphogenesis | 5 | portal protein, MW:57498 | 50% | 58% | 50% | 51% | 33% | 56% | 58% | 55% | 53% | 43% | 25% | 24% | 25% | |||||
| 6 | head protein, MW:34367 | 21% | 14% | 13% | 12% | 19% | 54% | 53% | 47% | 85% | 30% | 18% | 17% | 24% | ||||||
| 7 | scaffold protein, MW:21262 | 34% | 94% | 96% | 77% | 85% | 54% | 99% | 99% | 73% | 52% | 11% | 59% | 47% | 51% | 44% | ||||
| 8 | head protein, MW:12720 | 41% | 96% | 96% | 98% | 100% | 86% | 88% | 91% | 100% | 89% | 50% | 77% | 66% | 63% | 55% | ||||
| 9 | major capsid protein, MW:37491 | 10% | 20% | 81% | 85% | 82% | 82% | 59% | 83% | 84% | 81% | 82% | 37% | 62% | 61% | 65% | 63% | |||
| 10 | unknown function, MW:5997 | 89% | 53% | |||||||||||||||||
| 11 | unknown function, MW:13021 | 31% | 61% | 50% | 47% | 54% | 37% | |||||||||||||
| 12 | unknown function, MW:11470 | 66% | 73% | 68% | 70% | 58% | 64% | 39% | 58% | 35% | 30% | 30% | 45% | |||||||
| Tail Morphogenesis | 13 | unknown function, MW:12495 | 49% | 35% | 35% | |||||||||||||||
| 14 | unknown function, MW:14637 | 19% | 26% | 24% | 32% | 32% | 24% | 24% | 28% | |||||||||||
| 15 | major tail protein, MW:18525 | 26% | 84% | 90% | 86% | 86% | 64% | 88% | 86% | 88% | 86% | 53% | 66% | 60% | 65% | 64% | ||||
| 16 | unknown function, MW:13138 | 39% | 100% | 98% | 93% | 84% | 58% | 50% | 66% | 48% | 30% | |||||||||
| 17 | major tail protein, MW:12613 | 43% | 42% | 23% | 36% | |||||||||||||||
| 18 | minor tail protein, MW:153506 | 9% | 12% | 8% | 17% | 14% | 20% | 14% | 14% | 2% | 8% | 5% | 10% | |||||||
| 19 | minor tail protein, MW:57710 | 18% | 20% | 6% | 22% | 18% | 28% | 15% | 23% | 17% | 7% | 15% | ||||||||
| 20 | antireceptor, MW:177330 | 18% | 22% | 14% | 16% | 5% | 22% | 29% | 25% | 34% | 2% | 5% | 7% | 18% | ||||||
| 21 | minor tail protein, MW:74279 | 11% | 10% | 7% | 5% | 4% | 6% | 4% | ||||||||||||
| 22 | unknown function, MW:14539 | 73% | 31% | 82% | 90% | 77% | 84% | 20% | 44% | |||||||||||
| 23 | unknown function, MW:5475 | 45% | 47% | 47% | 47% | 47% | 47% | |||||||||||||
| Host Lysis | 24 | unknown function, MW:12328 | 33% | 33% | 38% | 33% | 38% | 28% | 23% | |||||||||||
| 25 | holin, MW:12004 | 31% | 75% | 74% | 72% | 59% | 39% | |||||||||||||
| 26 | lysin, MW:21754 | 49% | 48% | 38% | 39% | 49% | 44% | 35% | 31% | 20% | 29% | 42% | ||||||||
| Lysogeny Remnant | 30 | unknown function, MW:5249 | ||||||||||||||||||
| 31 | cro-like repressor, MW:7850 | 92% | 84% | 56% | 42% | 54% | 46% | 46% | ||||||||||||
| 32 | unknown function, MW:5039 | 85% | 58% | |||||||||||||||||
| Replication | 33 | unknown function, MW:18072 | 53% | 65% | 64% | 52% | 60% | 32% | 32% | 43% | 52% | |||||||||
| 34 | unknown function, MW:26164 | 28% | 21% | 47% | 46% | 66% | 36% | 22% | 35% | 44% | ||||||||||
| 35 | helicase, MW:50968 | 9% | 6% | 10% | 9% | |||||||||||||||
| 36 | unknown function, MW:17290 | 82% | 99% | 99% | 89% | 82% | 48% | 88% | 77% | 88% | 93% | |||||||||
| 37 | replication protein, MW:30474 | 29% | 15% | 28% | 33% | 16% | 16% | |||||||||||||
| 38 | primase, MW:59060 | 9% | 34% | 40% | 15% | 36% | 24% | 11% | 20% | 30% | ||||||||||
| *Transcriptional Regulation | 39 | unknown function, MW:12133 | ||||||||||||||||||
| 40 | unknown function, MW:9580 | 25% | 37% | 52% | 25% | |||||||||||||||
| 41 | unknown function, MW:6311 | |||||||||||||||||||
| 42 | DNA binding protein, MW:19572 | 23% | 41% | 18% | 52% | 54% | 54% | 48% | 57% | |||||||||||
| 43 | unknown function, MW:12132 | 37% | 35% | 26% | 24% | 26% | ||||||||||||||
| 44 | unknown function, MW:27652 | 47% | 86% | 83% | 45% | 72% | 31% | 52% | 56% | 41% | ||||||||||
Values are percent sequence coverages determined by dividing the number of amino acids detected in the mass spectrometry run by the total number of amino acids in a given protein. Numbers are averages between two technical replicates. Phage functional modules are labeled on the right [18]. * Orf1 is part of the transcriptional regulation module. Intron regions are not included in the figure (orf27–29) and orf 3 and orf4 encode one protein due to a splicing event [18].