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. 2012 May 30;7(5):e38103. doi: 10.1371/journal.pone.0038103

Figure 8. Effects of PA on cytoskeletals system of HUVECs.

Figure 8

(A) HUVECs were treated with PA, paclitaxel, cytochalasin B, or medium alone (untreated control) for 16 h. HUVECs were fixed and stained with DY554-phalloidin for F-actin, and anti-tubulin antibody for microtubules, respectively. Images were acquired on Cellomics ArrayScan HCS Reader. Number of (B) F-actin (stress fiber), and (C) Microtubule fibers count were analyzed by Cellomics Morphology BioApplication. Data are expressed as means ± SEM of three independent experiments. Statistical significance is expressed as ***, P<0.001; **, P<0.01; *, P<0.05 versus untreated control. Scale bar indicates 50 µm.