Figure 4. GST-GGA3 PBD binding to ARF6-GTP.

Myometrium tissue isolated from non-pregnant woman was lysed and the lysate was incubated with 0.1 mM GTPγS or 1 mM GDP for 30 min. The glutathione resin coupled to GST-GGA3 PBD was added to the lysate and continued the incubation for further 2 h. After washing the beads, the protein bound to the beads were analysed by immunoblotting using an anti-ARF6 mouse monoclonal antibody. The lysates that were not incubated with beads were used as input controls in immunoblotting.