Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 May 30;7(5):e37954. doi: 10.1371/journal.pone.0037954

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Kanamarlapudi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A. Analysis of the ARF1-GTP and ARF6-GTP levels in human placenta and myometrium. Lanes 1, Placenta and 2, NP myometrium. B. The GST-pulldown analysis of active ARF1 and ARF6 levels in human myometrium of NP, NIL, SL, NP-SL and PT-NIL. The tissue lysates from NP, NIL, SL, PT-NIL and PT-SL were incubated with GST-GGA3 PBD resin to precipitate ARF1-GTP or ARF6-GTP as described in the materials and method section. The total ARF1 and ARF6 expression in the cell lysates (upper panel) and the activated ARF1 and ARF6 that was pulled down with GST-GGA3 PBD (lower panel) were detected by immunoblotting using an anti-ARF1 rabbit polyclonal and an anti-ARF6 mouse monoclonal antibody respectively (i). The intensity of the bands was quantified by densitometric scanning. The data were expressed as percentage of total ARF1 or ARF6 that is GTP bound. The histogram represents an average of 4 samples for each condition (ii).