Table 1. The primer sequences used for RT-PCR analysis of ARF6, CYTH3 and GAPDH mRNA expression in the human myometrium.

Gene	Accession number	Forward primer Reverse primer Amplimer location	AmplifiedPCR productsize (bp)	References
ARF1	M36340	5′-GCCAGTGTCCTTCCACCTGTC-3′ 5′GCCTCGTTCACACGCTCTCTG-3′ 50–385	336	[44]
ARF6	NM_001663	5′-ATGGGGAAGGTGCTATCCAAA-3′ 5′-GCAGTCCACTACGAAGATGAGACC-3′ 622–891	270	[45]
CYTH1	M85169	5′-TAGCTAATGAAATTGAAAACCTGGGAT-3′ 5′-TTCATGGCAATGAACCTCTCCACAGTG-3′ 200–722	523	[28]
CYTH2	X99753	5′-CAGTGAAGCCATGAGCGAGGT-3′ 5′-TTCATGGCCACAAAGCGCTCCAGG-3′ 127–650	524	[28]
CYTH3	NM_004227	5′-ATCGACAATCTAACTTCCGTA-3′ 5′-ATGGCGATGAACCGTTCTGCCG-3′ 258–766	509	[28]
CYTH4	NM_013385	5′-TGTTTGCCCAAATCGACTGCT-3′ 5′-TCATCCACCTTCTGCACCGAG-3′ 246–1060	815	[28]
GAPDH	NM_002046	5′-ACCACAGTCCATGCCATCAC-3′ 5′-TCCACCACCCTGTTGCTGTA-3′ 586–1037	452	[46]

The starting and end position of the mRNA sequences amplified by RT-PCR are numbered (amplimer location) according to the sequences in the NCBI data base.