Figure 4. Nuclear localization of GPS2 and DIPA is affected in 3T3-L1 cells over-expressing Flag-ANKRD26-C.

A) 3T3-L1 cells transfected with GPS2-Myc (i–ix) and Flag-ANKRD26-C vectors (iv–ix) were double stained with antibodies against anti-Myc (i, iv, vii) and anti-Flag (ii, v, viii) and with DAPI solution for the nucleus staining (iii, vi, ix). B) 3T3-L1 cells transfected with DIPA-myc (i–ix) and Flag-ANKRD26-C vectors (iv–ix) were stained as in B. The images labeled iii, vi and ix are the merged fluorescent images. Note that only co-transfected cells in Panels vii, viii and ix demonstrate cytosolic localization on GPS2 and DIPA.