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. 2012 May 30;7(5):e37973. doi: 10.1371/journal.pone.0037973

Table 3. Antibodies used for immunofluorescent staining of duodenum biopsy tissues.

Antigen Cell Type/Component Isotype Working Manufacturer Catalog Number
Dilution or Clone
TV calicivirus TV-Infected (primary ab) Rhesus Plasma 1∶50 TNPRCa NAb
Rhesus IgG-FITCc TV-Infected (secondary ab) Goat IgGγ 1∶500 RDI-Fitzgerald RDI-617102012
Human Calnexin Endoplasmic Reticulum Rabbit IgG 1∶100 Santa Cruz SC-11397
Human Cytokeratin Epithelial Cell Mouse IgG1 1∶50 Biocare Medical CKLMW 8/18
Human Villin Intestinal Brush Border Rabbit IgG 1∶3 Cell Signalling Tech. 2369
Human CD3 T-lymphocyte Rabbit IgG 1∶100 Biocare Medical CP215C
Human CD20 B-lymphocyte Mouse IgG2a 1∶400 Dako M0755
Human IBA-1 Macrophage Mouse IgG1 1∶100 Santa Cruz SC-32725
Human CD11c Myeloid Dendritic Cell Mouse IgG 1∶10 Biosource 3.9
TUNEL (assay) Apoptotic Cell NA NA Millipore S7165
Human TG2 Tissue Transglutaminase 2d Mouse IgG1 1∶100 ThermoScientific MS-300-P1ABX
Nuclear DNA Nucleus NA 1∶1,000 Invitrogen T-3605
a

Tulane National Primate Research Center, bnot applicable, cFluorescein-Iso-Thio-Cyanate, dDigestive enzyme.