Table 3. Antibodies used for immunofluorescent staining of duodenum biopsy tissues.
Antigen | Cell Type/Component | Isotype | Working | Manufacturer | Catalog Number |
Dilution | or Clone | ||||
TV calicivirus | TV-Infected (primary ab) | Rhesus Plasma | 1∶50 | TNPRCa | NAb |
Rhesus IgG-FITCc | TV-Infected (secondary ab) | Goat IgGγ | 1∶500 | RDI-Fitzgerald | RDI-617102012 |
Human Calnexin | Endoplasmic Reticulum | Rabbit IgG | 1∶100 | Santa Cruz | SC-11397 |
Human Cytokeratin | Epithelial Cell | Mouse IgG1 | 1∶50 | Biocare Medical | CKLMW 8/18 |
Human Villin | Intestinal Brush Border | Rabbit IgG | 1∶3 | Cell Signalling Tech. | 2369 |
Human CD3 | T-lymphocyte | Rabbit IgG | 1∶100 | Biocare Medical | CP215C |
Human CD20 | B-lymphocyte | Mouse IgG2a | 1∶400 | Dako | M0755 |
Human IBA-1 | Macrophage | Mouse IgG1 | 1∶100 | Santa Cruz | SC-32725 |
Human CD11c | Myeloid Dendritic Cell | Mouse IgG | 1∶10 | Biosource | 3.9 |
TUNEL (assay) | Apoptotic Cell | NA | NA | Millipore | S7165 |
Human TG2 | Tissue Transglutaminase 2d | Mouse IgG1 | 1∶100 | ThermoScientific | MS-300-P1ABX |
Nuclear DNA | Nucleus | NA | 1∶1,000 | Invitrogen | T-3605 |
Tulane National Primate Research Center, bnot applicable, cFluorescein-Iso-Thio-Cyanate, dDigestive enzyme.