Figure 4. The interaction between RuvA_Mge and RecU_Mge on HJs.

(A) HJ-binding by RecU_Mge. The DNA-binding reactions were performed in a similar fashion as described in Fig. 3. Reactions were performed in volumes of 10 μl and contained 12.3 nM HJ 1.1 and the indicated concentrations of RecU_Mge. The positions of unbound HJs (HJ) and RecU_Mge-HJ complexes are depicted at the right-hand side of the gel. (B) The binding of RuvA_Mge to RecU_Mge-HJ complexes. RecU_Mge (0.5 μM) was incubated with HJ 1.1, followed by the addition of RuvA_Mge (at different concentrations, as indicated above the lanes). The nature of the various protein-DNA complexes is indicated at the right-hand side of the gel; RuvA_Mge-HJ complexes are indicated with a dot (•). (C) The binding of RecU_Mge to RuvA_Mge-HJ complexes. RuvA_Mge (4 μM) was incubated with HJ 1.1, followed by the addition of RecU_Mge (at various concentrations, as indicated above the lanes). The labeling of the figure is similar to that shown in (B).