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Copper XANES spectra of Methylocystis sp. strain M pMMO. The spectra for (A) reduced, (B) as-isolated, (C) Cu-reconstituted, membrane-bound, and (D) oxidized samples are shown. The reduced, as-isolated, and oxidized samples were solubilized. The dashed vertical line at ca. 8984 eV identifies spectral features corresponding to the CuI 1s → 4p transition. Spectra are offset for clarity.
