Copper EXAFS fitting analysis for Methylocystis sp. strain M pMMO. Raw EXAFS data for (A) reduced, (B) as-isolated, (C) Cu-reconstituted, membrane-bound, and (D) oxidized pMMO are shown in black. The reduced, as-isolated, and oxidized samples were solubilized. The phase shifted Fourier transforms of the copper data for (E) reduced, (F) as-isolated, (G) Cu-reconstituted, membrane-bound, and (H) oxidized are shown in black. Best-fit simulations are shown in gray.