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. 2012 May 30;7(5):e38157. doi: 10.1371/journal.pone.0038157

Figure 4. Production of IL-6, IL-10 and IL-12 in the culture supernatants of macrophages.

Figure 4

A. Macrophages from naïve and EAN mice were cultured with PBS or stimulated with LPS (100 ng/ml) plus IFN-γ (100 IU/ml) for 24 h. Supernatants were collected for ELISA detection. Without stimulation, macrophages from TNF-α KO mice (both naive and EAN mice) produced higher levels of IL-6 than those from WT mice, whereas after stimulation, macrophages from TNF-α KO mice produced lower levels of IL-6 than those from WT mice. B. For IL-12, the cytokine level in cultures of stimulated macrophages of TNF-α KO mice with EAN was significantly lower than WT mice with EAN. The level in cultures of stimulated macrophages was also lower in the control group than in the EAN group (for both WT and TNF-α KO mice). C. For IL-10, an opposite result to IL-12 was registered, i.e. stimulated and unstimulated macrophages from TNF-α KO mice with EAN produced higher levels of IL-10 than those from WT mice with EAN. As for IL-12, naïve mice showed no significant differences. Data are presented as mean value ± SD of one representative out of three independent experiments (n = 5 or 6 in each group). * p<0.01.