Figure 5. NO production of cultured macrophages.

PEMs from naïve and EAN mice were cultured and stimulated with LPS (100 ng/ml), IFN-γ (100 IU/ml), IL-12 (10 ng/ml), poly I:C (50 µg/ml), combinations thereof. In each set of stimulation, NO production was lower in the TNF-α KO group than in the WT group (p<0.01 for all comparisons). Data are presented as mean value ± SD of one representative out of three independent experiments (n = 5 or 6 in each group).