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. 2012 May 30;7(5):e38187. doi: 10.1371/journal.pone.0038187

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Nuñez Oreza et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Membrane vesicles (1 µg) containing high amounts of wild type ArcB^1–778 (circles) or the mutant ArcB variants (ArcB^L80V (squares) and ArcB^L87V (diamonds)) were incubated at room temperature with 40 µM [γ-³²P]ATP in the presence of 5 mM DTT (open symbols) or 250 µM Q0 (closed symbols) in a 20 µl reaction mixture. At the indicated time intervals a 5 µl sample was withdrawn for SDS-PAGE analysis. Left panel: autoradiograms of the gels. Right panel: net increase of ArcB-P with time, as quantitated with a PhosphorImager.