Figure 1. Assessment of cytosolic Ca²⁺ signaling in MDA-MB-468 and MDA-MB-231 breast cancer cell lines.

Average ATP-stimulated Ca²⁺ entry response (100 µM ATP), store-operated Ca²⁺ entry response (10 µM CPA) and non-stimulated Ca²⁺ influx (DMSO control) were assessed in A) MDA-MB-468 and B) MDA-MB-231 cells. Quantitation of peak relative Ca²⁺ influx (peak 2) in C) MDA-MB-468 and D) MDA-MD-231 cells; shown as average ± S.D. (n = 9 for MDA-MB-468 & n = 4 for MDA-MB-231 cells). E) MDA-MB-468 cells exhibit spontaneous and asynchronous Ca²⁺ oscillations that are attenuated by extracellular Ca²⁺ chelation (BAPTA). F) Still image showing the Y-plane section and G) the Y-plane projection through time. See also Movie S1. Representative of five movies from three independent experiments.