Figure 3. Agonist-stimulated and store-operated Ca²⁺ entry in MDA-MB-468 cells with EGF-induced EMT.

Average cytosolic Ca²⁺ response, peak relative Ca²⁺ influx (peak 2) and ratio of Ca²⁺ influx amplitude (peak 2) divided by AUC (t = 0–705 s) mediated by 100 µM ATP (A–C), 30 nM trypsin (D–F) and 10 µM CPA (G–I). Average time to peak (t = 0–705 s) for J) ATP, K) trypsin and L) CPA. Values show mean ± S.D. for nine wells from three independent experiments; * P<0.05 (unpaired t-test).