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. 2012 May 30;7(5):e36923. doi: 10.1371/journal.pone.0036923

Figure 8. Effect of TRPC1 and ORAI1 silencing on constitutive ERK1/2 activity and cell proliferation.

Figure 8

A) Representative immunoblot showing constitutive phosphorylation and total expression of ERK1/2 in MDA-MB-468 cells with ORAI1 or TRPC1 silencing. B) Densitometric data was obtained from the pooled data (three independent immunoblots) and shows ERK1/2 phosphorylation relative to total ERK1/2 expression; * P<0.05 (one-way ANOVA with Bonferroni post-tests). C) DAPI (cell number) and EdU staining (showing cells in S-phase of the cell cycle). D) Quantitation of the average cell count and (E) EdU positivity for nine wells from three independent experiments.* P<0.05 (unpaired t-test). All graphs show mean ± S.D. Scale bar represents 100 µm.