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. Author manuscript; available in PMC: 2012 May 30.
Published in final edited form as: Virology. 2005 Sep 15;342(2):240–251. doi: 10.1016/j.virol.2005.08.011

Fig. 7.

Fig. 7

Pulldown of proteins from 150 μg of walleye cell nuclear extracts by GST fusion proteins and glutathione sepharose. (A) Western blot of pulldown from W12 cell nuclear extract by wild type (wt) and mutated (V260S) GST-rv-cyclin AD (240–270) probed with antibodies that crossreact with walleye homologs of p300 and Sur2. (B) Pulldowns from nuclear extracts prepared from regressing walleye dermal sarcomas by wild type (wt) GST-rv-cyclin AD (240–270) and mutated forms, V260S and V260F and by VP16 subdomain fusions, GST-VP16N and GST-VP16C and GST-VP16 AD and corresponding mutated forms, F442S, F475A, and double mutation, 442S/475A, in the VP16 AD. ‘GST’ indicates pulldown with purified GST only. Input represents 2.5% of total extract used for each pulldown. 2 μg each of nuclear extracts from W12 cells (WNE) and HeLa cells (HNE) was loaded in the last two lanes and demonstrates crossreactivity of antisera to walleye proteins, p300 and Sur2.