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(a) Sucrose-density gradient sedimentation of virus from culture supernatants. WDSV-specific RNA sequence was measured by qRT-PCR and plotted against the gradient fraction density. (b) TEM micrograph of virus pellet. Magnification, ×300 000. (c) Amplicons of the 354 bp gag sequence from 1 μg DNA from RTgill cells infected with STEC virus pellet (STEC) or WDS tumour homogenate (WDS). Controls comprised 20 pg WDSV clone DNA (Plasmid) and 1 μg RTgill DNA. M, Molecular mass marker.
