Fig. 3.

Lethality resulting from rv-cyclin expression in injected zebrafish embryos during the first 4 days of development. a Percentage of lethality in embryos injected with CMVtk rv-cyclin at 250 µg/ml. Krt8 rv-cyclin (b) DNA was injected at 500 and 100 µg/ml and percentages of nonsurviving embryos recorded. All embryos were co-injected with a GFP reporter construct to monitor for successful injection and scored over the first 4 days of development. Control embryos were co-injected with a promoter-deletion plasmid (Δptr) and a GFP reporter plasmid. Data are presented as percentage of lethality of 200 injected embryos