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. Author manuscript; available in PMC: 2012 May 30.
Published in final edited form as: Mar Biotechnol (NY). 2010 Mar 28;13(2):142–150. doi: 10.1007/s10126-010-9274-2

Fig. 4.

Fig. 4

Expression of rv-cyclin in embryos co-injected with Krt8GFP. a Expression of GFP in the skin of co-injected embryos. b Expression of GFP in injected chimeric embryos correlates with rv-cyclin protein expression detected by western blot in zebrafish at 3 days, 1 month, 2 months, and 3 months of age. Immunohistochemistry of caudal tail transverse sections (40×-dorsal side up) from adult stable transgenic zebrafish (F1 offspring from founder 26) for Krt8-GFP and CMVtk rv-cyclin (c, d), treated with control antiserum (c) or rv-cyclin antibody (d). GFP expression in green, DAPI staining in blue, and rv-cyclin expression in red