Table 1.
Primers used for mutational analysis and SNPs identification in PTEN (89 613 715–89718 382 bp), NCBI Build 35
| PCR ID | Forward primer (5′-3 ′) | Reverse primer (5′-3 ′) | PCR product (bp) | Annealing temperature |
|---|---|---|---|---|
| 1 | AAGGGGGAATCTCTAGGCAA | AGCATGCCCAGTGTAGCTG | 453 | 62 |
| 2 | AGCAAGTGCAGCTGCAGG | AGAGGCTGCACGGTTAGAAA | 579 | 62 |
| 3 | CTCTCAGCGCCTGTGAGC | GGACTTGGCGGTAGCTGAT | 638 | 62 |
| 4 | CCAGTCGCTGCAACCATC | TGCAACCAGGCAAGAGTTC | 473 | 68 |
| 5 | CTGTCCATGTGGAAGTTACCTT | CTGTATCCCCCTGAAGTCCA | 460 | 62 |
| 6 | CCATAGAAGGGGTATTTGTTGG | CATGAATCTGTGCCAACAATG | 420 | 58 |
| 7 | AAAGATTCAGGCAATGTTTGTT | ATGCTGCACTTTAGTCTTCCTG | 350 | 60 |
| 8 | TTTCTCTGGAATCCAGTGTTTC | CAGATCCAGGAAGAGGAAAGG | 413 | 60 |
| 9 | GCTACGACCCAGTTACCATAGC | CCAATGAGTTGAACAACAAAGC | 404 | 60 |
| 10 | TTGCTTGAGATCAAGATTGCA | CTCACCAATGCCAGAGTAAGC | 446 | 60 |
| 11 | TTTTGTTGACTTTTTGCAAATG | GTCAAGCAAGTTCTTCATCAGC | 442 | 62 |
| 12 | AAGAGGGTCATTTAAAAGGCC | TCTGACACAATGTCCTATTGCC | 453 | 60 |
| 13 | CCAGAGAATGAACCTTTTGATG | ACCCTTCGGAAACCTCTCTTAG | 520 | 68 |
| 14 | GTTCTGTCACCAACTGAAGTGG | AGCATCCACAGCAGGTATTATG | 679 | 63 |