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. 1988 Apr 11;16(7):3075–3089. doi: 10.1093/nar/16.7.3075

Cloning and characterization of a gene from Rhizobium melilotii 2011 coding for ribosomal protein S1.

J Schnier 1, S Thamm 1, R Lurz 1, A Hussain 1, G Faist 1, B Dobrinski 1
PMCID: PMC336453  PMID: 3368316

Abstract

A 7 kb chromosomal DNA fragment from R. melilotii was cloned, which complemented temperature-sensitivity of an E. coli amber mutant in rpsA, the gene for ribosomal protein S1 (ES1). From complementation and maxicell analysis a 58 kd protein was identified as the homolog of protein S1 (RS1). DNA sequence analysis of the R. melilotii rpsA gene identified a protein of 568 amino acids, which showed 47% identical amino acid homology to protein S1 from E. coli. The RS1 protein lacked the two Cys residues which had been reported to play an important role for the function of ES1. Two repeats containing Shine-Dalgarno sequences were identified upstream of the structural gene. Binding studies with RNA polymerase from E. coli and Pseudomonas putida located one RNA-polymerase binding site close to the RS1 gene and another one several hundred basepairs upstream. One possible promoter was also identified by DNA sequence comparison with the corresponding E. coli promoter.

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Selected References

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  1. Adhya S., Gottesman M. Control of transcription termination. Annu Rev Biochem. 1978;47:967–996. doi: 10.1146/annurev.bi.47.070178.004535. [DOI] [PubMed] [Google Scholar]
  2. Bagdasarian M., Lurz R., Rückert B., Franklin F. C., Bagdasarian M. M., Frey J., Timmis K. N. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene. 1981 Dec;16(1-3):237–247. doi: 10.1016/0378-1119(81)90080-9. [DOI] [PubMed] [Google Scholar]
  3. Chow L. T., Kahmann R., Kamp D. Electron microscopic characterization of DNAs of non-defective deletion mutants of bacteriophage Mu. J Mol Biol. 1977 Jul 15;113(4):591–609. doi: 10.1016/0022-2836(77)90224-8. [DOI] [PubMed] [Google Scholar]
  4. Christiansen L., Pedersen S. Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural gene for ribosomal protein S1. Mol Gen Genet. 1981;181(4):548–551. doi: 10.1007/BF00428751. [DOI] [PubMed] [Google Scholar]
  5. Chu Y. G., Cantor C. R. Segmental flexibility in Escherichia coli ribosomal protein S1 as studied by fluorescence polarization. Nucleic Acids Res. 1979;6(6):2363–2379. doi: 10.1093/nar/6.6.2363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cole P. E., Sawchyn I., Guerrier-Takada C. Q beta replicase containing altered forms of ribosomal protein S1. J Biol Chem. 1982 Nov 10;257(21):12929–12934. [PubMed] [Google Scholar]
  7. Isono K., Isono S. Lack of ribosomal protein S1 in Bacillus stearothermophilus. Proc Natl Acad Sci U S A. 1976 Mar;73(3):767–770. doi: 10.1073/pnas.73.3.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kamen R., Kondo M., Römer W., Weissmann C. Reconstitution of Q replicase lacking subunit with protein-synthesis-interference factor i. Eur J Biochem. 1972 Nov 21;31(1):44–51. doi: 10.1111/j.1432-1033.1972.tb02498.x. [DOI] [PubMed] [Google Scholar]
  9. Kimura M., Foulaki K., Subramanian A. R., Wittmann-Liebold B. Primary structure of Escherichia coli ribosomal protein S1 and features of its functional domains. Eur J Biochem. 1982 Mar;123(1):37–53. doi: 10.1111/j.1432-1033.1982.tb06495.x. [DOI] [PubMed] [Google Scholar]
  10. Kitakawa M., Isono K. An amber mutation in the gene rpsA for ribosomal protein S1 in Escherichia coli. Mol Gen Genet. 1982;185(3):445–447. doi: 10.1007/BF00334137. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pedersen S., Skouv J., Kajitani M., Ishihama A. Transcriptional organization of the rpsA operon of Escherichia coli. Mol Gen Genet. 1984;196(1):135–140. doi: 10.1007/BF00334105. [DOI] [PubMed] [Google Scholar]
  14. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanger F., Coulson A. R., Barrell B. G., Smith A. J., Roe B. A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. doi: 10.1016/0022-2836(80)90196-5. [DOI] [PubMed] [Google Scholar]
  16. Schnier J., Faist G. Comparative studies on the structural gene for the ribosomal protein S1 in ten bacterial species. Mol Gen Genet. 1985;200(3):476–481. doi: 10.1007/BF00425734. [DOI] [PubMed] [Google Scholar]
  17. Schnier J., Isono K. The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1. Nucleic Acids Res. 1982 Mar 25;10(6):1857–1865. doi: 10.1093/nar/10.6.1857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schnier J., Kimura M., Foulaki K., Subramanian A. R., Isono K., Wittmann-Liebold B. Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1008–1011. doi: 10.1073/pnas.79.4.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schnier J., Stöffler G., Nishi K. Deletion and insertion mutants in the structural gene for ribosomal protein S1 from Escherichia coli. J Biol Chem. 1986 Sep 5;261(25):11866–11871. [PubMed] [Google Scholar]
  20. Stüber D., Bujard H. Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci U S A. 1981 Jan;78(1):167–171. doi: 10.1073/pnas.78.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Subramanian A. R., Mizushima S. Characterization of a mutant form of ribosomal protein S1 from Escherichia coli. J Biol Chem. 1979 Jun 10;254(11):4309–4312. [PubMed] [Google Scholar]
  22. Subramanian A. R., Rienhardt P., Kimura M., Suryanarayana T. Fragments of ribosomal protein S1 and its mutant form m1-S1. Localization of nucleic-acid-binding domain in the middle region of S1. Eur J Biochem. 1981 Oct;119(2):245–249. doi: 10.1111/j.1432-1033.1981.tb05600.x. [DOI] [PubMed] [Google Scholar]
  23. Subramanian A. R. Structure and functions of ribosomal protein S1. Prog Nucleic Acid Res Mol Biol. 1983;28:101–142. doi: 10.1016/s0079-6603(08)60085-9. [DOI] [PubMed] [Google Scholar]
  24. Thomas J. O., Kolb A., Szer W. Structure of single-stranded nucleic acids in the presence of ribosomal protein S1. J Mol Biol. 1978 Aug 5;123(2):163–176. doi: 10.1016/0022-2836(78)90319-4. [DOI] [PubMed] [Google Scholar]
  25. Yokota T., Arai K., Kaziro Y. Studies on 30S ribosomal protein S1 from E. coli. I. Purification and physicochemical properties. J Biochem. 1979 Dec;86(6):1725–1737. doi: 10.1093/oxfordjournals.jbchem.a132693. [DOI] [PubMed] [Google Scholar]
  26. Yokota T., Arai K., Kaziro Y. Studies on 30S ribosomal protein S1 from E. coli. II. Functional properties. J Biochem. 1979 Dec;86(6):1739–1745. doi: 10.1093/oxfordjournals.jbchem.a132694. [DOI] [PubMed] [Google Scholar]
  27. van Dieijen G., van Knippenberg P. H., van Duin J. The specific role of ribosomal protein S1 in the recognition of native phage RNA. Eur J Biochem. 1976 May 1;64(2):511–518. doi: 10.1111/j.1432-1033.1976.tb10330.x. [DOI] [PubMed] [Google Scholar]

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