Table 4.
Concentration values obtained from the proposed and published methods for Hcy analysis in real samples.
| Sample | Added (μM) | Expected (μM) | Proposed method (μmol L−1) | Published method (μmol L−1) | F ex | F tab, (0.05);2,2 | t ex | t tab (98%) |
|---|---|---|---|---|---|---|---|---|
| Urine | — | — | Less than limit of detection | Less than limit of detection | — | — | — | — |
| 5.00 | 5.00 | 4.85 ± 0.31 | 5.42 ± 0.51 | 5.8 | 19 | 2.3 | 3.8 | |
| 5.00 | 10.00 | 10.21 ± 0.44 | 10.48 ± 0.60 | 6.2 | 19 | 2.5 | 3.8 | |
| 20.00 | 30.00 | 30.12 ± 0.25 | 30.33 ± 0.41 | 5 | 19 | 2.0 | 3.8 | |
| Serum | Less than limit of detection | Less than limit of detection | — | — | — | — | ||
| 6 | 50.00 | 50.00 | 50.63 ± 0.65 | 50.87 ± 0.92 | 8.6 | 19 | 3.5 | 3.8 |
| 7 | 20.00 | 70.00 | 70.55 ± 0.58 | 70.67 ± 0.75 | 7.0 | 19 | 3.0 | 3.8 |
F ex: calculated F value; reported F value from F-test table with 95% confidence level and 2/2 degree of freedom; t ex: calculated t; t tab (98%): reported t value from student's t-test table with 98% confidence level.