Table 3.
Sample | rf (nN/s) | f* (pN) | koff (s−1) | xβ (nm) | xβ mean (nm) |
xβa mean (nm) |
R2b | |
---|---|---|---|---|---|---|---|---|
Tn | 0.64 | 34 | 1.2 ± 0.27 | 0.52 ± 0.03 | 0.79 | |||
1.8 | 42 | 4.0 ± 0.67 | 0.37 ± 0.02 | 0.85 | ||||
3.6 | 40 | 4.7 ± 0.79 | 0.45 ± 0.02 | 0.37 | 0.34 | 0.94 | ||
7.1 | 49 | 16.1 ± 2.0 | 0.28 ± 0.01 | 0.89 | ||||
c | 14 | 78 | 10.6 ± 2.6 | 0.23 ± 0.02 | 0.74 | |||
39 | 256 | 57 ± 13 | 0.02 ± 0.004 | 0.02 | 0.79 | |||
Tri | 0.18 | 40 | 0.64 ± 0.8 | 0.30 ± 0.16 | 0.56 | |||
0.63 | 42 | 0.13 ± 0.1 | 0.65 ± 0.008 | 0.95 | ||||
1.4 | 38 | 2.7 ± 1.0 | 0.43 ± 0.05 | 0.40 | 0.96 | |||
2.8 | 39 | 2.2 ± 0.8 | 0.55 ± 0.04 | 0.88 | ||||
5.8 | 44 | 5.8 ± 1.0 | 0.45 ± 0.02 | 0.96 | ||||
11 | 48 | 11 ± 2.0 | 0.39 ± 0.017 | 0.96 | ||||
Asialo Tri | 1.7 | 50 | 3.0 ± 1.5 | 0.30 ± 0.05 | 0.92 | |||
4.7 | 54 | 0.57 ± 0.2 | 0.54 ± 0.03 | 0.34 | 0.96 | |||
9.0 | 53 | 10 ± 1.5 | 0.32 ± 0.01 | 0.98 | ||||
19 | 64 | 48 ± 16.1 | 0.19 ± 0.02 | 0.81 | ||||
Asialo Fd | 0.75 | 27 | 2.5 ± 1.1 | 0.56 ± 0.07 | 0.94 | |||
2.0 | 27 | 0.88 ± 0.4 | 0.93 ± 0.07 | 0.98 | ||||
4.0 | 34 | 19 ± 2.9 | 0.36 ± 0.022 | 0.55 | 0.98 | |||
8.0 | 39 | 27 ± 8.5 | 0.32 ± 0.007 | 0.95 | ||||
17 | 56 | 1.2 ± 1.7 | 0.56 ± 0.11 | 0.84 |
xβ from f* vs rf.
R2 = coefficient of determination
inner energy barrier has become the rate limiting.