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R9LF-1 is a tight-binding inhibitor of LF. LF incubated with fluorogenic substrate in the absence or in the presence of varying concentration of R9LF-1 at 37 °C. Initial velocities were measured according to the increase of fluorescence intensity. Data were plotted as rate being the function of R9LF-1 concentrations. Inhibition constant (Ki) was obtained by fitting the curve using GraFit 5.
