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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Appl Microbiol Biotechnol. 2012 Jan 25;94(4):1041–1049. doi: 10.1007/s00253-012-3893-7

Fig. 6.

Fig. 6

Inhibition of LF by R9LF-1 and R9LF-1(desHA). (a) Difference in inhibition of the LF proteolytic activity towards a MAPKK-based fluorogenic peptide by the two forms of R9LF-1. Fluorogenic LF substrate was incubated with LF (10 nM) and the two forms of R9LF-1 (20 nM). Initial velocity was calculated according to fluorescence intensity increase which was monitored by Tecan 200. (b) R9LF-1(desHA) has decreased activity in inhibiting LF in cell lysate. J774A.1 cell lysate was incubated with two forms of R9LF-1 at the presence of LF. MAPKK cleavage was monitored by western blot.