Transparent blastocoel roof. (A) In this animal pole view of E. coqui midgastrulae, the blastocoel roofs are transparent, allowing the interior cavity of the blastocoels to be visible. (B) A section through a G. riobambae late blastula, treated with Hoechst 33258 to stain cell nuclei, reveals the thin blastocoel roof (top) as a single cell thick epithelium. (C) In this enlargement of (B), the thin blastocoel roof extends over large, yolky cells.