Figure 1.

Recovery of infectious Bunyamwera virus from plasmid DNAs. The diagram represents the cytoplasm of a cell that has been transfected with six different plasmids (circles), three encoding the viral proteins and three encoding the antigenome segments of the virus. All of the plasmids contain promoters for the bacteriophage T7 RNA polymerase, which is supplied before infection of the cells with a vaccinia virus/T7 recombinant. The antigenomes are incorporated into RNPs, replicated to form genomic RNPs, and then bud into the Golgi apparatus before release from the cell.