Figure 1.

Recruitment of RNAi and heterochromatin components to Mmi1 targets. During vegetative growth the Mmi1 protein targets DSR-containing transcripts for degradation. Mmi1 recruits the RNAi complex RITS and the Clr4-methyltransferase complex CLRC to participate in the repression of meiotic genes, as depicted in the case of mei4. During meiotic induction Mmi1 is sequestered in the Mei2 dot, causing dissociation of the complexes and expression of DSR-containing meiotic genes. The anti-silencing protein Epe1 helps remove H3K9me.