Table 1.

Strains and plasmids

Strain or plasmid Strain	Relevant property	Reference
P. aeruginosa
PA14	Wild-type	[39]
PAO1	Wild-type	[40]
ZK2870	Wild-type	[12]
PAO1 lasR	Markerless lasR mutant derived from PAO1	[41]
PA14 lasR	TnphoA lasR mutant derived from PA14	[42]
ZK lasR	Markerless in-frame lasR deletion in ZK2870	This study
ZK pelA	Markerless pelA deletion in ZK2870	[11]
ZK pslD	Markerless pslD deletion in ZK2870	[11]
ZK lasI	Markerless lasI deletion in ZK2870	This study
ZK pelA lasR	Markerless lasR deletion in a pelA mutant of ZK2870	This study
ZK pslD lasR	Markerless lasR deletion in a pslD mutant of ZK2870	This study
ZK pqsH	Markerless pqsH deletion in ZK2870	This study
ZK tpbA	Markerless pqsH deletion in ZK2870	This study
ZK lasR	pqsA suppressor mutation in a lasR mutant of ZK2870	This study
pqsA::Tn
ZK lasR	pqsR suppressor mutation in a lasR mutant of ZK2870	This study
pqsR::Tn
E. coli
DH5α	F- φ80dlacZΔM15 Δ (lacZYA-argF) U169 deoR recA1 endA1 hsdR17(rK- mK+) phoA supE44 λ-thi-1 gyrA96 relA1	Invitrogen
SM10	Mobilizing strain, RP4 tra genes integrated in the chromosome, thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmr	[43]
S17-1/λpir	TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir	[43]
Plasmid
mini-CTX-lacZ	Chromosomal integration vector, TcR	[44]
pEX18 Tc	Allelic exchange suicide vector, TcR	[45]
pEX18.ΔlasR	Suicide vector with lasR in-frame deletion	[41]
pEX18.ΔlasI	Suicide vector with lasI in-frame deletion	[41]
pEX18.ΔtpbA	Suicide vector containing tpbA in-frame deletion	This study
pLM1	Tn5 delivery vector, GmR	[46]
pLG10	pqsA-E operon cloned in pUCP18, ApR	[24]
pRG10	pqsA-D operon cloned under control of Plac of pUCP18, ApR	This study
pRG11	Promoter region of pel cloned in mini-CTX-lacZ vector	This study
pUCP18	Parent vector of pLG10, ApR	[47]