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. 2012 Mar 16;5:14. doi: 10.1186/1754-6834-5-14

Table 2.

Primers used for gene deletions

Δhxk1for 5'-ATGGTTCATTTAGGTCCAAAGAAACCACAGGCTAGAAAGGGTTCCATGGCTTCGTACGCTGCAGGTCGAC-3'
Δhxk1rev 5'-TTAAGCGCCAATGATACCAAGAGACTTACCTTCGGCAATTCTTTTTTCGGGCATAGGCCACTAGTGGATCTG-3'
Δglk1for 5'-ATGTCATTCGACGACTTACACAAAGCCACTGAGAGAGCGGTCATCCAGGCTTCGTACGCTGCAGGTCGAC-3'
Δglk1rev 5'-TCATGCTACAAGCGCACACAAGGCGGCACCCACTCCGGAACCATCCTTGGGCATAGGCCACTAGTGGATCTG-3'
Δhxk2for 5'-ATGGTTCATTTAGGTCCAAAAAAACCACAAGCCAGAAAGGGTTCCATGGCTTCGTACGCTGCAGGTCGAC-3'
Δhxk2rev 5'-TTAAGCACCGATGATACCAACGGACTTACCTTCAGCAATTCTTTTTTGGGGCATAGGCCACTAGTGGATCTG-3'
Δylr446wfor 5'-ATGACAATTGAAAGCACTCTAGCTCGGGAATTAGAAAGCTTGATTTTACCTTCGTACGCTGCAGGTCGAC-3'
Δylr446wrev 5'-TTATTGAACTTGGTTGTCTGATTTGTTCAAGTAGGTGGCTATTGCAGCGCCGCATAGGCCACTAGTGGATCTG-3'

Homology to the hexo-/glucokinase genes, bold; homology to kanMX cassette, italic.