Table 1.

Identification and quantitative analysis of early salt-responsive proteins in wild type and OSRK1 transgenic rice roots

Spota	Protein Identification	Matched peptide	Sequence Coverage (%)	Mrb (Ex/Tr)	NCBI Accession Number	Fold changec				Protein ratiod (OSRK1/WT)	Express Graphe
						WT		OSRK1
						3 h	7 h	3 h	7 h
Translation and transcription
5i	Elongation factor Tu	9	26.3	43.7/48.4	gi|21685576	ndf	7.32	2.47	1.19	Ig
12i	Putative translation elongation factor eEF-1 beta chain	5	35.3	30.0/23.8	gi|113612079	0.97	2.00	1.92	0.55	1.25
323	Glycine-rich RNA-binding protein	6	74.0	13.1/15.5	gi|115441831	1.02	0.38	1.14	0.85	0.93
383	Translation initiation factor 5A	2	25.0	17.7/17.4	gi|113611710	nd	2.84	1.04	0.62	Ig
Amino acid and purine metabolism
26	Putative inosine monophosphate dehydrogenase	2	4.70	57.4/51.9	gi|215694434	0.38	1.25	0.89	0.88	1.05
27	Aspartate-semialdehyde dehydrogenase	3	10.9	40.1/40.1	gi|113549818	2.11	3.05	1.15	0.80	5.46
67	Aspartate aminotransferase	4	13.0	40.6/44.8	gi|29468084	1.48	4.51	0.87	0.97	3.64
68	Putative isovaleryl-CoA dehydrogenase	1	2.70	41.5/44.5	gi|113578072	0.88	1.89	0.91	0.89	2.53
200	Glutamine synthetase	4	10.4	41.4/39.2	gi|124052115	1.51	1.37	0.98	1.02	1.72
259	Methylmalonate semi-aldehyde dehydrogenase	5	10.5	18.1/57.2	gi|113610618	0.46	0.60	1.22	0.67	0.27
314	Aspartate aminotransferase	6	18.8	42.2/44.9	gi|215768565	1.13	3.14	0.87	1.06	3.08
314	Glutamate dehydrogenase	7	21.4	42.2/44.3	gi|33242905	1.13	3.14	0.87	1.06	3.08
Detoxyfying enzymes
51	Glutathione reductase, cytosolic	11	24.8	55.6/53.4	gi|113538016	0.63	2.80	0.89	1.01	1.82
112i	Gutathione S-transferase	4		25.6/25.6	gi|31433227	1.67	0.72	1.35	1.51	0.28
116	Ascorbate peroxidase	5	33.6	25.8/27.1	gi|50920595	1.79	1.13	0.80	1.10	2.40
125	Glutathione S-transferase II	6	32.1	26.0/24.0	gi|3746581	2.05	1.42	0.94	0.90	2.63
160i	Ascorbate peroxidase	10	46.0	24.0/27.1	gi|1321661	1.35	0.38	1.51	0.71	0.41
198	Quercetin 3-O-methyltransferase	2	6.8	41.4/39.7	gi|113623000	1.57	1.19	1.06	0.84	1.83
200	Peroxidase	3	8.6	41.4/37.8	gi|257657027	1.51	1.37	0.98	1.02	1.72
202	Similar to glyoxalase II	6	28.2	29.9/33.1	gi|113533338	1.07	1.98	0.94	0.90	1.47
206	Glyoxalase I	10	51.5	33.9/32.5	gi|113623141	3.57	1.73	0.84	1.00	4.46
213	Thioredoxin Type H	7	50.8	13.6/13.9	gi|82407383	1.94	0.87	1.01	0.98	3.20
265i	Similar to glyoxalase II	7	32.5	32.5/33.2	gi|113533338	0.59	1.52	0.68	0.74	1.90
310	Ascorbate peroxidase	5	38.2	25.6/27.1	gi|257707656	0.79	0.34	1.85	4.40	0.10
351	Ascorbate peroxidase	4	24.8	26.7/27.1	gi|1321661	1.98	0.65	1.00	0.97	1.35
390	Aldehyde dehydrogenase	3	8.0	54.6/59.2	gi|8163730	3.16	3.10	0.85	1.25	10.85
Glycolysis and other carbohydrate metabolism related proteins
61	Fructose -bisphosphate aldolase	3	9.8	29.3/38.8	gi|790970	0.26	0.94	0.69	0.81	0.82
67	Phosphoglycerate kinase	3	11.0	40.6/42.3	gi|113596357	1.48	4.51	0.87	0.97	3.64
76i	Dihydrolipoamide dehydrogenase precursor	5	13.5	60.6/52.6	gi|113532449	nd	16.87	0.87	1.54	Ig
77	Transketolase	7	10.6	76.8/80.0	gi|227468492	2.14	15.09	1.02	0.98	18.68
79	Similar to enolase	6	15.9	52.0/50.7	gi|115478881	1.90	0.78	0.80	0.74	3.14
116	Triose phosphate isomerase	13	79.1	25.8/27.6	gi|553107	1.79	1.13	0.80	1.10	2.40
275	2,3-Bisphosphoglycerate-independent phosphoglycerate mutase	8	27.2	70.8/60.8	gi|257353836	1.45	2.56	0.80	0.84	2.07
277	2,3-Bisphosphoglycerate-independent phosphoglycerate mutase	3	12.2	70.8/60.8	gi|257353836	1.26	2.83	0.86	0.88	2.33
279i	Enolase	4	14.4	57.0/47.9	gi|113548027	1. 03	1.70	0.72	0.80	1.41
292	Phosphoglyceromutase	9	20.8	69.9/60.7	gi|257353838	1.03	2.13	0.93	1.04	2.66
293	Phosphoglyceromutase	12	28.1	69.9/60.7	gi|257353838	1.50	1.79	0.87	1.02	1.90
315	Glyceralde-3-phosphate dehydrogenase	4	16.3	39.1/36.5	gi|968996	0.99	2.14	0.91	1.10	2.15
318	Glyceralde-3-phosphate dehydrogenase	4	16.3	38.7/36.4	gi|968996	1.17	2.68	1.03	1.01	3.06
353	Triose phosphate isomerise	4	20.9	22.0/27.0	gi|169821	0.83	0.62	1.16	0.67	0.32
385	Phosphoglycerate kinase	5	16.7	45.4/42.2	gi|113596357	nd	4.57	1.03	1.10	Ig
Proteolytic enzymes
112i	Proteasome subunit beta type 2	5	26.4	25.6/23.4	gi|17380213	1.67	0.72	1.35	1.51	0.28
134	Oryzacystatin	3	23.5	13.3/11.4	gi|1280613	2.10	0.46	0.87	1.00	0.22
204	Proteasome subunit alpha type 2	7	34.6	26.2/27.6	gi|259443357	0.59	1.88	0.89	1.15	1.22
351	Proteasome subunit alpha type 2	4	16.6	26.7/25.8	gi|259443327	1.98	0.65	1.00	0.97	1.35
Heat shock proteins
112i	Putative chaperonin21 precursor	5	26.2	25.6/26.3	gi|51090748	1.67	0.72	1.35	1.51	0.28
274	Chaperonin CPN60-1, mitochondrial precursor	10	17.5	64.1/61.0	gi|113547409	1.17	2.77	0.88	1.12	1.49
276i	DnaK-type molecular chaperone precursor	2	4.6	75.0/70.4	gi|257307253	1.26	3.35	0.76	1.00	2.78
278i	DnaK-type molecular chaperone precursor	6	14.5	72.9/70.4	gi|257307253	2.35	2.38	0.66	1.47	4.47
Lipid biosynthesis
258	Putative enoyl-ACP reductase	3	15.7	35.4/39.1	gi|113623526	1.32	2.41	1.13	1.01	2.92
69	Putative acetyl-CoA C-acetyltransferase	6	19.2	43.1/44.1	gi|113630918	1.28	3.46	0.80	0.99	6.40
82	Putative inorganic pyrophosphatase	5	21.4	31.8/33.0	gi|113537770	0.88	0.41	0.97	0.99	0.42
177	Nucleoside diphosphate kinase from rice	2	16.6	14.5/16.8	gi|113639936	0.39	0.81	0.88	0.83	1.14
227i	Putative inorganic pyrophosphatase	5	13.9	32.1/33.0	gi|113537770	1.70	0.41	0.92	1.30	0.44
Stress related proteins
124	Formate dehydrogenase, mitochondrial precursor	13	47.2	42.4/41.2	gi|21263611	0.64	7.24	0.78	0.90	4.13
134	15 kda Organ-specific salt-induced protein	2	23.4	13.3/15.2	gi|256638	2.10	0.46	0.87	1.00	0.22
214	15 kda Organ-specific salt-induced protein	7	77.2	13.4/15.2	gi|256638	4.05	0.33	0.87	1.12	0.48
227i	15 kda Organ-specific salt-induced protein	6	64.8	32.1/15.2	gi|256638	1.70	0.41	0.92	1.30	0.44
386	Pathogen-related protein	4	21.3	16.5/17.2	gi|16589076	4.29	2.28	1.30	0.38	6.70
Signal transduction related proteins
225	Calreticulin	3	5.9	33.4/47.9	gi|6682833	0.75	0.43	1.47	1.26	0.26
Unkwown proteins
392	Hypothetical protein	3	10.7	25.8/27.4	gi|14192878	5.65	4.00	0.79	0.96	18.95

^aSpot numbers correspond to the spots in Figure 2 and Figure 3

^bMolecular weight (kDa) of protein spots estimated from gel analysis (Ex) and theoretical molecular weight of identified protein (Tr)

^cFold change of each spot after NaCl treatment for 3 h or 7 h, calculated from the mean spot volumes in control and NaCl treated gel groups

^dSpot-volume ratio of WT to OSRK1, calculated from the mean spot volumes in 3 h control of WT and OSRK1 groups

^eRelative expression graphs of protein spots after NaCl treatment in WT and OSRK1. Spot volumes are analyzed by Progenesis software. The left four bars indicate spot volume of WT root and right four bars indicate spot volumes of OSRK1. From left to right, each bar indicate spot volume of 3 h control, 3 h NaCl treated, 7 h control and 7 h NaCl treated gel groups. Values are means ± SE. Error bars from three spots in three independent gels

^fnd: not detected

^g I: spots were not detected in WT groups, but appeared in OSRK1 groups

^hProteins not identified from the database

i Salt-responsive proteome identified from OSRK1 transgenic rice roots