Table 2.

The potential SnRK2 phosphorylation sites found in protein spots showing more than 1.5 fold change in OSRK1 transgenic rice roots at unstressed condition

Spota	Protein Identification	NCBI Accession #	Potential recognition motifs (SnRK2)b	Sc	T/Wd
5	Elongation factor Tu	gi|21685576	RRILS, RGIT	U	I
27	Aspartate-semialdehyde dehydrogenase	gi|113549818	RRPSS, FLRVIS	U	U
61	Fructose-bisphosphate aldolase	gi|790970	RFAS	D	D
67	Aspartate aminotransferase	gi|29468084	RVAT, RVKS	U	U
68	Putative isovaleryl-CoA dehydrogenase	gi|113578072	RRLYS, LVRHGS	U	U
69	Putative acetyl-CoA C-acetyltransferase	gi|113630918	RSSS, RKGS	U	U
76	Dihydrolipoamide dehydrogenase precursor	gi|113532449	RLGS, RFMT	U	I
77	Transketolase	gi|227468492	RNLS, RVVS	U	U
79	Simillar to enolase	gi|115478881	RAAT, RNQS, RMGS	U	U
82, 227	Putative inorganic pyrophosphatase	gi|113537770	RRRCSLRTNS, RKVS	D	D
112	Glutathione S-transferase	gi|31433227	Not found	U	D
112	Proteasome subunit beta type 2	gi|17380213	RRAYT	U	D
112	Putative chaperonin21 precursor	gi|51090748	RVCS, RRPS	U	D
116	Triose phosphate isomerise	gi|553107	Not found	U	U
124	Formate dehydrogenase, mitochondrial precursor	gi|21263611	Not found	U	U
125	Glutathione S-transferase II	gi|3746581	MARPSS	U	U
134	Oryzacystatin	gi|1280613	Not found	D	D
134, 214, 227	15 kda organ-specific salt-induced protein	gi|256638	FGRSGT	D	D
160	Ascorbate peroxidise	gi|1321661	Not found	D	D
200	Peroxidase	gi|257657027	IDRAKS, RNDS	U	U
200	Glutamine synthetase	gi|124052115	RTLS, RRLT, RHET, RGAS, RPAS	U	U
202, 265	Putative glyoxalase II	gi|113533338	RARPIS	U	U
206	Glyoxalase I	gi|113623141	IQRGPT	U	U
213	Thioredoxin type H	gi|82407383	Not found	U	U
225	Calreticulin	gi|6682833	RARSSS	D	D
258	Putative enoyl-ACP reductase	gi|113623526	IGRALS, RAMS, RVNT	U	U
259	Methylmalonate semi-aldehyde dehydrogenase	gi|113610618	LLRSGS, RVQS, RDAT, VKRASS	D	D
275,277	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	gi|257353836	RSET, VKRNKS	U	U
276, 278	DnaK-type molecular chaperone precursor	gi|257307253	FARTFS, RQAT, INRNTT	U	U
292, 293	Phosphoglyceromutase	gi|257353838	RIAS, RAET, VKRNKS	U	U
314	Glutamate dehydrogenase	gi|33242905	LTRVFT	U	U
314	Aspartate aminotransferase	gi|215768565	RLPT	U	U
315, 318	Glyceralde-3-phosphate dehydrogenase	gi|968996	RAAS, RVPT	U	U
385, 67	Phosphoglycerate_kinase	gi|113596357	Not found	U	U
383	Translation initiation factor 5A	gi|113611710	RLPT	U	I
386	Pathogen-related protein	gi|16589076	Not found	U	U
390	Aldehyde dehydrogenase	gi|8163730	RRGSS, LQRFST, RVGT	U	U
392	Hypothetical protein	gi|14192878	VLRLRS, RYAT	U	U

^aSpot numbers correspond to the spots in Figure 2

^bPotential phosphorylation motifs for SnRK2 kinase, RXX(S/T) were analyzed. Motifs with favouring amino acids (L, I, V, M, F, R) at -5 position for CDPK/SnRK2 were marked as bold characters according to Vlad et al. (2008). Among these, the highly preferred motifs, (L/I)XRXX(S/T) were underlined

^cUp (U) or down (D)-regulated protein spots in wild type rice roots after NaCl treatment for 3 h or 7 h

^dUp (U) or down (D)-regulated or induced (I) protein spots in OSRK1 transgenic rice roots compared to wild type roots at 3 h or 7 h control