Figure 3.

In silico analysis of reaction essentiality between P. pastoris, P. stipitis, and S. cerevisiae cultivated in minimal media with glucose. Reactions from GEMs of the three yeasts were deleted one by one and growth was maintained as the objective function. Reactions were classified according to the resulting phenotype; essential is where cells stop to grow after the deletion (red); partially-essential means a reduction in growth rate (blue); and non-essential when no effect on growth was seen (green). Reactions were arrangement in pathways for a better comparison.